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Lentinula edodes Consumption: Proliferation, Activation and Modification of Memory and Naive Innate Immune Cell Populations
Author(s) -
Stanilka Joy M,
Rowe Cheryl A,
Creasy Rebecca A,
Dai Xiaoshuang,
Percival Susan S
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.643.17
Subject(s) - mushroom , lentinula , immune system , peripheral blood mononuclear cell , biology , flow cytometry , cd3 , natural killer t cell , t cell , immunology , ex vivo , cd8 , in vivo , in vitro , food science , biochemistry , microbiology and biotechnology
A human nutrition intervention was conducted to evaluate the effect of dried Lentinula edodes (Shiitake) mushrooms on immunological changes in healthy subjects. 51 subjects were randomized to 1(50g) or 2 (100g) servings/d for 28d. Peripheral blood mononuclear cells were isolated and cultured for 24hr or 6d with PHA‐L, before and after mushroom consumption. Proliferation and activation of gamma delta T (γδTCR+/CD3+), NK (CD56+/CD3−) and NKT (CD56+/CD3+) cells were determined by flow cytometry. In addition, naïve (CD45RA+) and memory (CD45RO+) γδT cell populations were determined. After mushroom consumption, significant increases in proliferation were observed after 24hr for NK and NKT cells and after 6d for all three cell types. An increase in the ability to proliferate ex vivo suggests a more robust response may occur in vivo . Changes in the distribution of CD45RO+ and CD45RA+ on γδ TCR+ cells were observed suggesting mushroom consumption influences maturation of γδ‐T cells in vivo . Expression of CD69 on γδ T cells and CD314 on NK and NKT cells was significantly increased during 24hr of culture, after 28d of mushroom consumption. Increased ability to express these activation markers suggests the cells may be primed. Overall, changes observed in these innate immune cells suggest positive benefits of regular Shiitake mushroom consumption. Support from US Mushroom Council & Australian Mushroom Growers Assn.