Premium
Inhibitory effects of resveratrol on IgE‐mediated degranulation and anaphylactic reaction in basophilic mast cells and mice
Author(s) -
Han SeonYoung,
Kang YoungHee
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.643.16
Subject(s) - resveratrol , degranulation , histamine , immunoglobulin e , chemistry , pharmacology , allergic response , inflammation , allergic inflammation , mast cell , immunology , syk , immune system , medicine , biochemistry , antibody , signal transduction , receptor , tyrosine kinase
Resveratrol is a phytoalexin abundantly found in red grape skin, and is effective in anti‐tumor and anti‐inflammation associated with immune responses. This study was conducted to test whether resveratrol suppressed IgE‐mediated allergic responses and passive cutaneous anaphylaxis (PCA) in RBL‐2H3 cells and in mice. RBL‐2H3 cells sensitized with anti‐DNP‐IgE and subsequently stimulated by DNP‐HSA increased the release of β– hexosaminidase and histamine. When RBL‐2H3 cells were pretreated with nontoxic resveratrol at 1–25 μM, such induction was dose‐dependently diminished. Syk, PLCγ, PKCμ and PKCθ of sensitized RBL‐2H3 cells were activated by stimulation with DNP‐HSA, which was dampened by ≥5 μM resveratrol. Furthermore, oral administration of 10 mg/kg resveratrol reduced serum histamine level enhanced in sensitized mice challenged to DNP‐HSA. Additionally, resveratrol decreased IgE‐mediated PCA and alleviated allergic edema in mice. Allergic skin inflammation, induction of MCP‐1 and MIP‐2, were inhibited by supplementing resveratrol to sensitized antigen‐challenged mice. Resveratrol inhibited mast cell‐derived immediate‐type allergic reactions, and these responses of resveratrol suggest possible therapeutic strategies in preventing allergic inflammatory diseases.