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The Influence of Type II Diabetes on the Immune Response of Obese Adults
Author(s) -
Paich Heather A.,
Sheridan Patricia A.,
Noah Terry L.,
Weir Samuel S.,
Beck Melinda A.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.643.13
Subject(s) - type 2 diabetes , diabetes mellitus , immune system , medicine , endocrinology , insulin , type 1 diabetes , glycolysis , cd28 , cd8 , inflammation , peripheral blood mononuclear cell , immunology , biology , metabolism , in vitro , biochemistry
We have previously shown that obesity impairs expression of CD4 + and CD8 + T cell markers of activation and function. Obesity is associated with type II diabetes; however, not all obese individuals develop type II diabetes. Type II diabetes is associated with low levels of chronic inflammation and a higher prevalence of infections. The objective of our study was to elucidate the differences in the human cellular immune response between obese individuals with type II diabetes and obese individuals without type II diabetes. We stimulated peripheral blood mononuclear cells, obtained from obese individuals with and without type II diabetes, ex vivo with live pandemic influenza virus A and anti‐CD3/anti‐CD28 beads. Markers of activation, function, and signaling were measured using flow cytometry. We also measured three parameters of cellular glycolysis, including glycolytic rate, glycolytic capacity, and glycolytic reserve capacity, in T cells stimulated with or without anti‐CD3/anti‐CD28 bead stimulation and with or without insulin stimulation. We found significant differences between obese individuals with and without type II diabetes, demonstrating that metabolic disruptions in insulin signaling further increase immune dysfunction in obese adults. Grant Funding Source : NIH ROI AI078090 and P30DK056350