Premium
In vivo regulation of gene transcription by alpha and gamma‐tocopherol in murine T lymphocytes
Author(s) -
Zingg JeanMarc,
Han Sung Nim,
Pang Eunice,
Meydani Mohsen,
Meydani Simin Nikbin,
Azzi Angelo
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.640.6
Subject(s) - t cell , gene expression , cd28 , microbiology and biotechnology , biology , lymphotoxin , gene , vitamin e , t cell receptor , cd40 , cytotoxic t cell , receptor , in vitro , immunology , biochemistry , immune system , antioxidant
Vitamin E is present in food in 8 different analogues (α‐, β‐, γ‐, δ‐tocopherols and tocotrienols). Although alpha‐tocopherol (α‐T) has been mostly studied, the other analogs also have raised interest, especially gamma‐tocopherol (γ‐T) which is abundant in the US diet. We compared the effect of dietary supplementation with adequate or high doses of α‐T or γ‐T on induction of gene expression in activated T cells. Old C57BL/6 mice were fed diets containing adequate (30 PPM) or high (500 PPM) doses of α‐T or γ‐T for 4 weeks, T cells from the spleen isolated and stimulated with plate‐bound anti‐CD3 and soluble anti‐CD28, and gene expression changes assessed by gene array analysis. The data obtained indicate significant qualitative and quantitative differences between the two analogs in regulating gene expression induced by T cell stimulation. Unique marker genes were found that could be useful for establishing the response to vitamin E for both α‐T (e.g. induced: CD40 ligand, lymphotoxin A) and γ‐T (e.g. repressed: poliovirus receptor‐related 2) supplementation. Interestingly, with high doses of supplementations, α‐T activated a higher number of genes than γ‐T, whereas γ‐T reduced a higher number of genes than α‐T in stimulated T‐cells. Whether the observed changes in gene expression in T cells after high γ‐T supplementation reduces overall inflammation or results in changes in T cell mediated function remains to be determined. Supported by USDA Contract #58–1950‐0–014.