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Oral administration of nutrients recovers intestinal functions in total parenteral nutrition (TPN) model of rats
Author(s) -
Suzuki Takuji,
Kasahara Ayako,
Keta Ayumi,
Sato Ayaka,
Mayanagi Yuki
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.640.4
Subject(s) - nutrient , digestion (alchemy) , parenteral nutrition , stimulation , disaccharidase , jejunum , medicine , ileum , absorption (acoustics) , biology , endocrinology , chemistry , biochemistry , small intestine , ecology , physics , chromatography , acoustics
It has been considered that influx stimulation of nutrients in intestinal tract is important for keeping the intestinal functions. Therefore, we investigated whether the influx stimulation of nutrients through the intestinal tract recover the intestinal function using intestinal dysfunction model of rats. We performed total parenteral nutrition (TPN) against 8 weeks old rats for 7 days (TPN7). After 7days, rats were administered respective nutrients orally, i.e. carbohydrates, amino acids and lipids, for 3 days. We collected jejunum, and analyzed expression levels of digestion/absorption of nutrients related genes by qRT‐PCR and measured disaccharidase activity. Although the expression of digestion/absorption of nutrient related genes markedly increased by lipids and carbohydrates admin, the enzyme activities did not change. These results suggested the expression levels of nutrients digestion/absorption related gene were recovered by nutrients for 3 days, but it needed more time to express the intestinal function by nutrient admin orally. In conclusion, our data suggest that influx stimulation of nutrients to intestinal tract is important to keep on the intestinal functions, and fructose and medium‐chain triglyceride may be important for recovery of intestinal function.