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Effect of early life Ca and P restriction on renal transporters gene expression in post weaning piglets
Author(s) -
Graugnard Daniel E,
Spry Malinda L,
Samuel Ryan S,
Jolliff James S,
Mahan Donald C,
Brennan Kristen M
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.640.22
Subject(s) - weaning , biology , zoology , medicine , gene expression , endocrinology , andrology , gene , biochemistry
The objective of this study was to evaluate the effect of Ca and P restriction during the post‐weaning period in pigs. Piglets (n=5/treatment) were assigned to one of 4 treatments: Control (100% NRC requirements), or 7‐d, 14‐d or 21‐d postweaning Ca and P restriction (33% of NRC requirements). After restriction periods, piglets were fed 100% of NRC requirements. Performance was measured until d 35, at which time kidney gene expression of renal transporters was measured using qPCR relative to the control group. Performance did not differ between treatments. However, during d 21–35 the gain‐to‐feed ratio was greater ( P < 0.05) in piglets restricted for 21 d (0.692 G:F) or 14 d (0.672 G:F) compared with 7 d (0.583 G:F). Relative mRNA level of the solute carrier family 34 member 1( SLC34A1 ) was up‐regulated ( P < 0.05) in piglets restricted for 21 d (1.16‐fold) or 14 d (1.18‐fold), consistent with improved gain‐to‐feed ratio and indicating improved active phosphate transport into cells. The solute carrier family 8 member 1 ( SLC8A1 ) was down‐regulated ( P < 0.05) in piglets restricted for 7 d (−1.78‐fold), demonstrating a long‐term effect of early life nutrition on Ca metabolism. No differences were observed in ATP2B1, SLC22A and SLC34A2 mRNA levels. These results suggest performance and renal capacity benefits in response to 14 to 21 d post‐weaning Ca and P restriction. Grant Funding Source : Alltech, Inc.