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Bioaccessibility of carotenoids from transgenic provitamin A biofortified sorghum
Author(s) -
Lipkie Tristan E,
De Moura Fabiana F,
Zhao ZuoYu,
Albertsen Marc C,
Che Ping,
Glassman Kimberly,
Ferruzzi Mario G
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.638.20
Subject(s) - sorghum , biofortification , carotenoid , food science , germplasm , amaranth , carotene , biology , sweet sorghum , bioavailability , dry weight , micronutrient , microbiology and biotechnology , chemistry , agronomy , bioinformatics , organic chemistry
Biofortified sorghum lines are being developed to target vitamin A deficient populations in Sub‐Saharan African. The purpose of this study was to screen diverse samples of transgenic sorghum for the content and delivery of provitamin A carotenoids using an in vitro digestion model. The germplasm background and transgenic sorghum contained 1.0–1.5 and 3.3–14.0 μg/g β‐carotene equivalents on a dry weight basis (DW), respectively. Test porridges made from milled sorghum contained 16–250 μg/100g β‐carotene equivalents on a fresh weight basis. Micellarization efficiency of all‐E‐ β‐carotene was lower (p<0.05) from transgenic sorghum (0.9–4.9%) than from null/non‐transgenic sorghum (6.3–11.1%). ABS 188 sample #11 contained the greatest bioaccessible β‐carotene content (0.67±0.12μg/g DW) in relation to null/non‐transgenic samples (0.08–0.20 μg/g DW). While the bioavailability and bioconversion of provitamin A carotenoids from these grains must be confirmed in vivo , these data support the notion that biofortification can enhance the total and bioaccessible concentrations of β‐carotene in sorghum. Support provided by HarvestPlus, DuPont Pioneer, and a grant from the USDA National Needs Program.