Premium
Fructooligosaccharides augments the effect of a flavonoid, α‐ glucosyl‐isoquercitrin, on glucagon‐like peptide 1 (GLP‐1) secretion
Author(s) -
Phuwamongkolwiwat Panchita,
Hira Tohru,
Hara Hiroshi
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.637.34
Subject(s) - in vivo , glucagon like peptide 1 , medicine , endocrinology , chemistry , secretion , basal (medicine) , enteroendocrine cell , analysis of variance , endocrine system , type 2 diabetes , insulin , hormone , biology , diabetes mellitus , microbiology and biotechnology
Glucagon‐like peptide‐1 (GLP‐1), which is released from the enteroendocrine cells in the presence of glucose, has shown to improve glucose tolerance. Fructooligosaccharides (FOS) and α‐ glucosyl‐isoquercitrin (Q3GM) also improve glucose metabolism but its effect on the role of GLP‐1 is unclear and warrants further investigation. We used two groups of rats (i.e., conscious and anesthetized) to conduct in vivo and in situ experiments, respectively, to investigate the secretion of GLP‐1 post administering Q3GM with‐ and without FOS. Baseline blood samples were obtained via jugular vein for in vivo but portal vein for in situ rat group with subsequent samples obtained at 15, 30, 60, 90 and 120 minutes to evaluate plasma GLP‐1 levels by using ELISA. We examined the differences among groups with 2‐way ANOVA with post‐hoc Tukey‐Kramer's test for GLP‐1 level among the group and Dunnett's for the differences from the basal blood sample. We found that for both in vivo and in situ experiments, Q3GM without FOS stimulated the secretion of GLP‐ 1, transiently. On the contrary, Q3GM with FOS significantly enhanced and prolonged plasma GLP‐1 concentrations increased by Q3GM in both in vivo and in situ experiments. Our results suggest that Q3GM with FOS has the potential for prevention or treatment of type 2 diabetes by enhancement of GLP‐1 secretion and prolongation the high plasma concentration of the antidiabetic hormone. Grant Funding Source : Hokkaido University