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Glucose and cyanidin‐3‐glucose interrupt quercetin metabolism in HepG2 cells
Author(s) -
Hashimoto Naoto,
Blumberg Jeffrey,
Chen CY. Oliver
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.636.19
Subject(s) - quercetin , metabolism , chemistry , biochemistry , enzyme , intracellular , superoxide dismutase , catalase , carbohydrate metabolism , antioxidant
The biological activity of flavonoids is principally exerted by their metabolites formed via phase II enzymes. The activity of these enzymes is subject to the influence of genetic, physiological, and dietary factors. We examined the effect of glucose (Glc) and the anthocyanidin cyanidin‐3‐glucose (C3G) on metabolism of the flavonol quercetin (Q) in human hepatic carcinoma HepG2 cells in vitro . Cells were treated with Glc (5.5, 30, 50 mmol/L), C3G (1, 10, 25 μmol/L) or their combination for 3 d, followed by incubation with 30 μmol/L Q for 4–24 h. Q metabolites in media and cell lysates were assessed using HPLC‐UV. Maximum production of Q metabolites by HepG2 cells treated with 5.5 mmol/L Glc was noted in cell lysate at 4 h and all intracellular Q metabolites excreted to the media at 16 h. Production of Q metabolites was inhibited in a Glc and C3G dose‐dependent manner. The combination of 50 mmol/L Glc plus 25 μmol/L C3G also decreased production of total Q metabolites due largely to the inhibitory effect of Glc. The addition of superoxide dismutase, catalase or their combination to the media containing 50 mmol/L Glc did not ameliorate the reduction in the production of Q metabolites. Thus, Q metabolism may be down‐regulated by hyperglycemia and C3G; further research is warranted to elucidate the mechanism of this action. Support by NARO and USDA.