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Caging Influences Tissue Concentrations of Vitamin K in Rodents
Author(s) -
Shen Xiaohua,
Shea M. Kyla,
Booth Sarah,
Callahan Michael F,
Loeser Richard,
Fu Xueyan
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.635.13
Subject(s) - feces , medicine , vitamin , endocrinology , biology , chemistry , zoology , ecology
Animal models used in vitamin K (VK) studies require VK tissue manipulation while maintaining adequate coagulation. To investigate this in a rodent model used for the study of VK on cartilage, male Sprague Dawley rats (3mo, n=20) were fed either a VK‐deficient diet [21.9±3.9 μg phylloquinone (PK)/kg, D group] or control diet (1035±131μg PK/kg, C group) following destabilization of the medial meniscus knee surgery. Bleeding (in feces) was observed in D group only when housed in wire bottomed cages for 3–5 d. The bleeding stopped once moved to conventional cages. Fecal samples (n=5 group) were obtained at 3.5 wk. In D group, PK concentrations were only 2% of that in C group. No menaquinone‐4 (MK4) was detected in feces in either group whereas MK6–13 concentrations were present in both (p=0.49). At 6 wk, no MK4 was detected in either D or C group in serum or liver; PK was detected in C group only. Kidney PK and MK4 concentrations were 3.4±2.6 and 39.1±3.7nmol/kg, respectively in D group, which were 24% and 50% of their respective values in C group. Our data suggest that conventional cages used with a VK deficient diet reduces PK and MK‐4 concentrations in tissues while avoiding abnormal bleeding. The biological availability of long chain MKs from feces is not known.