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Plasma glutathione resettlement by oral glutamine supplementation is associated with glycine normalization in HIV+ treated patients
Author(s) -
Burini Roberto Carlos,
BorgesSantos Maria Doroteia,
Moreto Fernando,
MingYu Yong
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.631.2
Subject(s) - glutathione , glutamine , chemistry , medicine , glycine , amino acid , endocrinology , biochemistry , enzyme
HIV+ patients present low concentrations of reduced GSH and its precursors. To investigate the responses of the GSH pathway to Gln dietary supplements in 12 HIV+ treated patients (6 men and 6 women, 22 – 45 years old) and 20 healthy controls (Co, 10 men and 10 women, 20 – 59 years old) were studied at their usual diet (Do) and after 7‐day dietary‐supplements containing glutamine (Gln, 20g/d). Blood samples were drawn after overnight fast and HPLC plasma analysis of amino acids, total (GSH) and oxidized glutathione (GSSG) were carried out at moments before (M0) and after the intervention period (M1). Statistical comparisons were made between groups (Co X HIV+) with p<0.05. Under Do the HIV+ group showed higher GSSG along with lower concentrations of GSH and all amino acids except Hcy. Met, Gly and GSH were normalized by the dietary Gln. The 71.4% increase of GSH in Gln group at M1 was followed by the increase of its precursors markedly Gly (27.2%) and slightly by Glu (13.7%) and Cys (10.8%), nevertheless without detectable reduction in the GSSG/GSH ratio. Moreover the intervention led to an increased Tau/Cys (18.9%) and decrease of Glu/Gln (6.6%) ratios relatively to Co. In conclusion, the restoration of GSH normal levels by Gln was markedly supported by Gly replenishment. The restricted contribution of Cys and Glu to GSH generation could be due to either their limited formation (Gln→Glu) and/or higher utilization (Cys→Tau). Supported by FAPESP, CAPES, CNPq