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LOW GLUTAMINE CONCENTRATION IS OBSERVED IN EQUINE TESTIS
Author(s) -
Filho Helio Cordeiro Manso,
Silva Rodrigues Armele Karina,
Manso Eliana C.C.,
Lima Ewerton B,
Manso Helena Emilia C.C.,
Wanderley Erika K.,
Abreu Jose Mario Girao
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.631.19
Subject(s) - glutamine , semen , glutaminase , glutamine synthetase , metabolism , andrology , diluent , medicine , biology , chemistry , endocrinology , biochemistry , amino acid , nuclear chemistry
Glutamine (gln) is frequently added to equine semen as a diluent to improve spermatozoa life, however there is little information about concentration of glutamine metabolism in equine testis. Gln and Glu concentrations were determined, by enzymatic analyses, in regions near the testicular vein (TA), near the external area of the testis (TB), the head of the epididymus (EH), and the tail of epididymus (ET). Samples were obtained from stallions (n=10) that underwent surgery to remove the testes. ANOVA and Tukey's test were used analyze the results, with P<5%. Tissues were washed to remove the semen present before analysis. Results showed significant diference in [glu+gln] between all four tissues. Lower [glu+gln] was observed in in EH (5.781±1.073 umol/mg of tissue) when compared with other 3 areas (ET: 10.970 ± 0.782; TA: 13.272±0.671; TB:10.840 ±1.463) (P<0.05). Overall, this work indicates considerable differences in glutamine plus glutamate concentrations in different regions of the testis. However to determine more precise the glutamine metabolism in all four tissues we need to determine GS and glutaminase activity in these tissues, and a definitive interpretation can be presented.