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Effect of hypercaloric diet on RAGE mRNA expression, oxidative stress and kidney function in rats
Author(s) -
Pierine Damiana Tortolero,
Luvizoto Renata Azevedo Melo,
Francisqueti Fabiane Valentini,
Ferreira Ana Lucia Anjos,
Yeum KyungJin,
Correa Camila Renata
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.630.4
Subject(s) - rage (emotion) , glycation , oxidative stress , medicine , endocrinology , renal function , kidney , messenger rna , receptor , pathogenesis , inflammation , biology , gene , biochemistry , neuroscience
Advanced glycation end products (AGEs) produced under oxidative stress can trigger porinflammatory responses by interacting with receptors for AGEs (RAGE). RAGE may link to the pathogenesis of chronic kidney diseases. Objective To evaluate the influence of hypercaloric diet on RAGE mRNA expression and oxidative stress in the kidney and its physiologic function. Methods Male Wistar rats were fed a control diet (C, n=14) or a hypercaloric diet (HD, n=14) for 12 weeks. The adiposity index (AI) was used as an obesity indicator. Kidney function was evaluated by glumerular filtration rate, kidney RAGE mRNA expression by real time PCR, and kidney oxidative stress by total antioxidant performance (TAP). Results RAGE mRNA expression was significantly (p<0,001) higher in HD (1.89±0.77) than C rats (0.76±0.37). A positive correlation between RAGE and AI (r=0.64, p <0.001) was found. There were no differences in glomerular filtration rate and TAP between groups. Conclusion The hypercaloric diet for 12 weeks resulted in an increase of RAGE mRNA expression, which may lead to inflammation in the kidneys from HD group. Support: CAPES, FAPESP (2010/06100–9)