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Effects of Agrimonia pilosa Ledeb. on insulin resistance induced in high fat diet fed rats
Author(s) -
Lee Young Min,
Nam Song Yee,
Kim Young Sub,
Jang Hwan Hee,
Kim Sang Mi,
Kim Mi Ju,
Kim Haeng Ran
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.630.14
Subject(s) - insulin resistance , chemistry , insulin , medicine , apigenin , postprandial , adipose tissue , endocrinology , biochemistry , biology , flavonoid , antioxidant
The purpose of this study is to investigate the effects of aqueous extract from A. pilosa on insulin resistance induced by high fat diet in rats. Using liquid chromatography‐mass spectrometry, the 3 major peaks of A. pilosa aqueous extract were identified as apigenin‐hexose, luteolin‐glucuronide, and apigenin‐glucuronide. Male Sprague Dawley rats were assigned to the following groups (n=10): normal fat diet (NF); high fat diet (HF); high fat diet with 0.1% A. pilosa aqueous extract (HFA). Experimental diets were fed for 16 weeks. In our results, high fat diet significantly increased liver and epididymal fat weight compared with NF group (p<0.05), however there was a significant decrease in liver weight by supplementation of A. pilosa aqueous extract. In oral glucose tolerance test at 15 week, we observed the blood glucose levels of the HFA group were lower than those of the HF group at 30, 60, 120 min after glucose administration (p<0.05). In addition, dietary A. pilosa aqueous extract increased serum adiponectin concentration compared with HF group (p<0.05). The mRNA expression of gluconeogenesis was decreased in HFA group (p<0.05). These data suggest that A. pilosa aqueous extract can improve insulin resistance in rats fed on high fat diet. This study was carried out with the support of the Research Program for Agricultural Science & Technology Development (Project No. PJ008554012012).