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Human milk components inhibit H1N1 influenza virus infection in vitro
Author(s) -
Liu Bo,
Yu Zhuoteng,
Newburg David S.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.629.2
Subject(s) - lactoferrin , in vitro , mucin , virus , virology , microbiology and biotechnology , biology , luciferase , chemistry , biochemistry , gene , transfection
Breastfed infants have a lower risk of influenza than those weaned early, suggesting that human milk could contain components that inhibit infection by influenza virus. This study was to test whether human milk per se inhibited H1N1 pseudovirus infection in vitro, and to identify the milk components responsible for any inhibition. The inhibition model was Pseudovirus (A/California/04/2009, H1N1) infection of cultured Madin‐Darby Canine Kidney (MDCK) cells. The luciferase plasmid of this pseudovirus allowed infection to be quantified via a luciferase assay. Inhibition was measured for pooled human milk and its fractions, including lactoferrin, mucin 1, mucin 4, human milk oligosaccharides (HMOS), and bile salt stimulated lipase (BSSL). At 80 μg/mL of Lactoferrin, 100 μg/mL of mucin 1, 150 μg/mL of mucin 4 and 50 μg/mL HMOS, these components inhibited H1N1 influenza virus infection in vitro . Thus, multiple milk components at their native concentrations have the ability to protect against H1N1 influenza virus, and perhaps other types of influenza viruses. Grant Funding Source : NIH HD013021 , AI075563 , Abbott Nutrition