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Comparison of the sialic acid components of mature human milk in three countries
Author(s) -
Burgher Anita M.,
Li Xi,
RamirezMayans Jaime A.,
OntijoBarrios Ericka M.,
Sablan Benjamin,
Wali Yassar,
Van Alstine Leslie,
Pramuk Kathryn
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.629.18
Subject(s) - sialic acid , lactation , zoology , chemistry , food science , biology , biochemistry , pregnancy , genetics
The objective of this study was to evaluate the relationship between the total sialic acid, 3′‐sialyllactose (3SL), 6′‐sialyllactose (6SL), and free sialic acid (SA) of mature human milk and stage of lactation in mothers from the Philippines, Oman and Mexico. Milk samples were obtained from 176 healthy mothers from Mexico, Oman and the Philippines. who were exclusively breast feeding infants (1–4 months old). Anion exchange chromatography with pulsed amperometric detection (HPAEC‐PAD) was used to quantify 3SL, 6SL, and free SA in all human milk samples. Total SA was quantified after an acid hydrolysis sample treatment. Method validations were conducted to confirm the precision and accuracy for all analytes. Results Total SA, Free SA and SA from 3′‐sialyllactose and 6′‐sialyllactose in human milk (Mean, mg/L)Lactation Stage 28–30 days 31–60 days 61–90 days 91–115 days All samplesTotal Sialic Acid 866 619 484 359 525 SA from 6′SL 226 135 97 51 108 SA from 3′SL 60 59 55 48 55 Free SA 7 9 11 11 10 Other SA 573 417 322 249 354Conclusions The predominant trend demonstrated using an Analysis of Covariance with Lactation Stage in days as the covariate showed that for all measures except the Free Sialic Acid, there is a statistically significant decrease in the amount of Sialic Acid in human milk as Lactation Stage increases (p‐values < 0.0001). This research was funded by Pfizer Nutrition.