Premium
Pheophorbide‐a prevents vascular endothelium dysfunction and down‐regulates AGEs‐induced proinflammatory cytokines.
Author(s) -
Nam MiHyun,
Oh JiSun,
Hong Chungoui,
Son DaHee,
Hong SeungTeak,
Seol HaeMin,
Lee KwangWon
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.594.2
Subject(s) - proinflammatory cytokine , pheophorbide a , inflammation , endothelium , chemistry , glycation , monocyte , tumor necrosis factor alpha , rage (emotion) , cytokine , reactive oxygen species , pharmacology , endocrinology , medicine , biochemistry , biology , receptor , organic chemistry , photodynamic therapy , neuroscience
During hyperglycemia, nonenzymatic glycation occurs through the reaction of aldehyde groups of reducing sugars and free amino groups of proteins. Excessive intracellular reactive oxygen species (ROS) derived from advanced glycation end products (AGEs) may play a key role of inflammation on endothelium. ROS enhance the expression of specific adhesion molecules on the surface of endothelium, and it up‐regulates the monocyte adhesiveness. Glycapaldehyde induced AGEs (Glycol‐AGEs) have been reported to express pro‐inflammatory cytokines such as tumor necrosis factor‐alpha (TNF‐α) and interleukin 1‐beta (IL‐1β), and it might be one of the important factors in the pathogenesis of initial stage of atherosclerosis. Pheophorbide a showed not only a scavenging activity of the intracellular ROS, but also monocyte adhesiveness inhibitory activity on THP‐1 and HUVEC co‐cultivation system. The mRNA levels of inflammation related genes such as MCP‐1 and IL‐6 were significantly decreased by pheophorbide a, and AGEs‐stimulated TNF‐α and IL‐1β were down‐regulated with it. In these results, we demonstrated that the pheophorbide a has a significant ROS scavenging activity, monocyte adhesive inhibitory activity, and down regulation activity of cytokines related to inflammation on endothelium. We expect that the pheophorbide a might potent candidate compound to control endothelial cell dysfunctions.