Premium
Characterization of Riboflavin Binding Protein structure
Author(s) -
Alazawi Neam K,
Smith Sheila R,
Benore Marilee Ann
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.590.9
Subject(s) - recombinant dna , western blot , biochemistry , chemistry , binding protein , riboflavin , vitamin , retinol binding protein , transport protein , binding site , retinol , gene
Riboflavin‐Binding Protein (RBP) is a glycophosphoprotein responsible for the transport of the vitamin Riboflavin in oviparous and other species. Evidence of copper binding by RBP suggests that the protein plays a role in copper transport. Identification and characterization of the specific RBP:Copper binding site is hindered by the lack of assay methods for the native or recombinant proteins. An improved protocol to purify and identify RBP was developed using Western blot methods adapted and optimized using rabbit antibody for identification. These methods can be used for purification and identification of recombinant RBP proteins.