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Fluorescent nanoparticles based on fluorescein
Author(s) -
Araujo Ana Paula Vieira,
Gomes Anderson J
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.575.5
Subject(s) - zeta potential , fluorescein , tetraethyl orthosilicate , dynamic light scattering , nanoparticle , fluorescence , materials science , scanning electron microscope , nanotechnology , chemical engineering , chemistry , optics , composite material , physics , engineering
This study aimed to demonstrate the characterization of the fluorescent compound fluorescein when encapsulated in Tetraethyl orthosilicate nanoparticles (TEOS). In this study, fluorescein was incorporated in TEOS nanoparticles using the sol‐gel process and these particles were evaluated in relation to size using dynamic light scattering (DLS), zeta potential, drug encapsulation efficiency, and external morphology using scanning electron (SEM) and spectroscopic properties. The fluorescein was loaded into TEOS nanoparticles, and the better entrapment efficiency was 75%. SEM and DLS revealed that the particles are spherical in shape, being the smallest size approximately 180 nm, and show low tendency to aggregate, confirmed by zeta potential ranging around of −39 mV and −50 mV. Those results suggest that TEOS nanoparticles can be used asociated to antitumoral drugs and locate tumor cells using fluorescence imaging assays. Financial support: CNPq, CAPES, FAPDF, FINATEC, DPP‐UnB