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Impacts of Silver Nanoparticles on Bacterial Multidrug Efflux Pump
Author(s) -
HE GUI,
Zhang Wei,
Chen Huizhong,
Saliba Sam,
Thorpe Conner,
Rogers Eugene,
Hsie Shufeng
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.575.12
Subject(s) - efflux , ciprofloxacin , ethidium bromide , enterobacter cloacae , chemistry , microbiology and biotechnology , silver nanoparticle , antibiotics , antibacterial activity , nuclear chemistry , pharmacology , bacteria , biochemistry , escherichia coli , medicine , biology , enterobacteriaceae , nanoparticle , materials science , nanotechnology , dna , genetics , gene
Although silver nanoparticles (NPs) has shown antibacterial function, however, its impacts on drug resistance are not clear. Here, we report the impacts of silver NPs on multidrug efflux pump (MDRP) of Enterobacter cloacae . Antibacterial tests were performed with CLSI broth dilution method. E. cloacae strains with and without MDRP, EmmdR were utilized. Also, ciprofloxacin, benzalkonium, ethidium bromide, and trimethoprim were utilized for susceptibility tests. The drug efflux alteration of EmmdR after exposure to silver NPs was determined. Also, we tested the antibiotic‐H + antiport alteration after exposure to silver NPs by using a fluorescence quenching assay. The expression of emmdR were measured by using a real‐time PCR method. Susceptibility tests showed a silver NPs (< 50nm) enhancing the inhibition of antibiotics such as ciprofloxacin and benzalkonium on the growth of E. cloacae. The efflux of ethidium bromide was decreased after exposure to silver NPs. The ciprofloxacin‐H + antiport activity was decreased after exposure to the silver NPs also. Also, the expression of emmdR did show a significant increase after exposure to the silver NPs. In conclusion, this study discovered that silver NPs is able to improve the antimicrobial effectiveness of antibiotics in E. cloacae. Also, the silver NPs affect expression and function of EmmdR in E. cloacae.