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Comparative proteomic analysis of an isogenic pair of lung normal and lung cancer cell line
Author(s) -
Alam Md Maksudul,
Hooda Jagmohan,
Cadinu Daniela,
Henke R. Michael,
Ma Xiaotu,
Zhang Li
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.573.2
Subject(s) - lung cancer , western blot , biology , cytoskeleton , proteomics , translation (biology) , actin cytoskeleton , cell culture , cancer research , pathology , cell , microbiology and biotechnology , medicine , genetics , messenger rna , gene
Lung cancer is the leading cause of cancer‐related deaths in both men and women. Non‐small cell lung cancer (NSCLC) represents ~85% of lung cancer cases. Understanding how NSCLC cells differ from normal lung cells can help divulge the underlying causes of this disease. In this study, we analyze the protein expression pattern of an isogenic pair of normal (HBEC30KT) and NSCLC (HCC 4017) lung cell line. iTRAQ‐based quantitative differential MALDI TOF MS/MS was performed on eight cancer (NSCLC) samples and eight normal controls. We have identified 1465 proteins, 282 of which were differentially expressed with 1.3‐ fold change, with 119 up‐regulated and 163 down‐regulated. The protein expression pattern was verified by western blot for some randomly selected proteins. The up‐regulated proteins are involved in various functions including ribosome function, protein translation, mitochondrial functions and so on. However, among the down‐regulated proteins, most of them are involved in cytoskeleton and cell motility. The predicted cytoskeletal structures were also examined; as predicted from proteomic data, the cancer cell line showed thin and short actin stress fibers with reduced focal adhesion.