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Correlation between Genotypes and Behavioral Phenotypes in a Mouse Model simulating aspects of Human Post‐traumatic Stress Disorder (PTSD)
Author(s) -
Srinivasan Seshamalini,
Sowe Bintu,
Miller StacyAnn,
Muhie Seid,
Chakraborty Nabarun,
Hammamieh Rasha,
Meyerhoff James,
Jett Marti
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.569.1
Subject(s) - amygdala , basolateral amygdala , phenotype , prefrontal cortex , correlation , hippocampus , genotype , psychology , neuroscience , gene , biology , cognition , genetics , geometry , mathematics
Biologically meaningful correlations between behavioral phenotypes and genotypes are evaluated in a modified ‘residentintruder’‐ type stress on mice. This model was established by housing male C57BL/6 naïve subject mice with an SJL aggressor (Agg) mouse without direct contact for 6 h/d for 10 d or 5 d, and then briefly pairing the C57BL/6 mice with the Agg mice for 3x/d. Controls (C), also housed without food/liquid during the 6hr session, did not experience direct physical contact with Agg. At time points of 24 hours or 6 wks after the last exposure, behaviors of C and aggressor‐exposed (Agg‐E) mice were studied using the partition test, which allows mutual exposure of only sensory cues without physical contact. Behavioral patterns (partition avoidance, freezing) of Agg‐E mice suggested fear and social withdrawal, the reported traits of PTSD. Global gene expression profiling of three major stress‐relevant brain regions, namely Hippocampus (HC), Amygdala (AY), and Medial Pre‐Frontal Cortex (mPFC) shows a clear genotype distribution between the C and Agg‐E in AY and not in HC which is a positive correlation with PTSD symptom severity. The cluster pattern of MPFC reflects similarity between the genotype profile and behavioral phenotype. Further comprehensive analysis can potentially lead to identifying diagnostic and therapeutic biomarkers that serve as internal indicators of the complex psycho‐pathogenesis.