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Ultra‐Sensitive Detection of Breast Cancer Stem Cell Specific Surface Marker CD44 based on a Polyvalent Directed Peptide Polymer
Author(s) -
Yoon MoonYoung,
Cho JunHaeng,
Lee SangChoon
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.568.2
Subject(s) - cd44 , chemistry , peptide , breast cancer , linker , cancer cell , antigen , cancer research , cancer , cell , microbiology and biotechnology , biochemistry , biology , medicine , immunology , computer science , operating system
Early detection of the cancer biomarkers have important clinical applications, improves the chances of efficient treatment and cure. CD44, a cancer‐associated membrane glycoprotein involved in cell adhesion and tumor progression, has been implicated as a cancer stem cell antigen in several cancers including breast cancer and thus early detection is crucial. Herein, we screened a peptide library and identified two novel diverse peptides that bind with high affinity to CD44 (KD of ~100 – 200 nM). Further, a polyvalent directed peptide polymer (PDPP), a flexible poly‐D‐lysine polymer conjugated with different target‐binding peptides through N‐succinimidyl‐3‐(2‐pyridyldithio)‐propionate linker, was designed to achieve higher binding affinity and sensitivity than the single peptides. The PDPP constructed with both of the identified peptides shown increased binding affinity (KD of ~18 nM) to CD44 than PDPPs constructed with individual peptides and detects a lower limit of 100 pg/ml. Our work demonstrates a basis to develop novel ultra‐sensitive PDPP as diagnostic probe which may replace antibodies as CD44 detection probes.