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Suppression of adipocyte differentiation and adipogenesis by extracts of sweet potato leaves
Author(s) -
Lin ChiaYu,
Chiang TzuYuan,
Yin MeiChin
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.562.1
Subject(s) - adipogenesis , adipocyte , oil red o , endocrinology , medicine , intracellular , cellular differentiation , 3t3 l1 , cell growth , chemistry , biology , biochemistry , adipose tissue , gene
Obesity is a major health concern due to its strong association with a number of diseases and metabolic abnormalities, including diabetes, dyslipidemia, and heart disease. Inhibition of adipocyte differentiation is an important strategy to inhibit obesity. In this study, we investigated the effects of sweet potato leaves (SPL) on the inhibition of adipocyte differentiation and adipogenesis in 3T3‐ L1 cells. SPL at 5mg/ml~0.5 mg/ml impaired 3T3‐L1 cell proliferation as indicated by MTT assay. To determine whether SPL reduces proliferation through cytotoxic effects, we measured LDH leakage into the culture medium in response to SPL treatment. SPL was found significantly to promote adipocyte cell death (P<0.05). To examine the effect of SPL on differentiation and adipogenesis, the postconfluent 3T3‐L1preadipocytes were exposed to differentiation medium in the presence or absence of SPL for 9 days. The inclusion of SPL in the media remarkably inhibited adipocyte differentiation and fat accumulation, evidenced by both Oil Red O staining and the measurement of intracellular triacylglycerol concentrations in a dose‐dependent manner (P<0.05). Moreover, SPL treatment also reduced ROS generation during adipocyte differentiation and adipogenesis. Our results suggest that SPL might be the potential agent for obesity therapies.