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Polarized release of TCR‐enriched microvesicles at the center of the T cell immunological synapse
Author(s) -
Choudhuri K.,
Llodrá J.,
Roth E. W.,
Tsai J.,
Gordo S.,
Wucherpfennig K. W.,
Kam L.,
Stokes D. L.,
Dustin M. L.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.553.24
Subject(s) - microvesicles , immunological synapse , microbiology and biotechnology , endosome , microvesicle , escrt , chemistry , synapse , t cell receptor , biology , intracellular , t cell , immunology , immune system , microrna , biochemistry , neuroscience , gene
Using high resolution optical and electron microscopy, we show that centrally accumulated T cell receptors (TCR) at in the center of the immunological synapse (IS) is located on the surface of extracellular microvesicles that bud at the IS center. An early endosomal‐sorting complex required for transport (ESCRT) sorts TCR for inclusion in microvesicles, while terminal ESCRT components mediate scission of microvesicles from the T cell plasma membrane. The HIV polyprotein GAG co‐opts this process for budding of virus‐like particles. B cells bearing cognate pMHC receive TCR from T cells and initiate intracellular signals in response to isolated synaptic microvesicles. These microvesicles deliver transcellular signals across antigen‐dependent synapses by engaging cognate pMHC on APC.