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DNA Based Folding Bio‐Sensors That Are Capable of Detecting Transcription Factors Involved In Cancer Progression By Expressing Recombinant TATA Binding Protein
Author(s) -
Reyna Yerelsy C,
Bonham Andrew J
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.551.11
Subject(s) - tata binding protein , transcription preinitiation complex , transcription factor ii a , tata box , transcription factor , computational biology , tata box binding protein , dna , transcription (linguistics) , recombinant dna , biology , gene , promoter , chemistry , microbiology and biotechnology , dna binding protein , genetics , gene expression , linguistics , philosophy
Transcription factors bind to specific genomic sequences to control the flow of genetic information from DNA to mRNA. The preinitiation complex of these proteins includes TATA binding protein (TBP), which specifically recognizes the TATA box, a highly conserved region at the beginning of many genes. As many cancers result from the aberrant regulation of transcription factors, there is great interest, both academically and diagnostically, in a better understanding these binding events, and TBP is a core factor that can help guide the design of novel probes of transcription. Here, we report on the design and use of DNA‐based folding biosensors that are capable of quantitatively and sensitively detecting TBP using custom optical and electrochemical probes that are activated by TBP's intrinsic DNA‐binding activity. These sensors will ultimately allow us to investigate the role that protein‐protein interactions and inhibitory drugs have on gene expression.