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Pyrimidine dimer glycosylase mediated repair of ultravioletinduced mtDNA damage
Author(s) -
Calkins Marcus J,
Lloyd R. Stephen,
McCullough Amanda K
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.545.3
Subject(s) - pyrimidine dimer , mitochondrial dna , mitochondrion , dna damage , dna repair , biology , ultraviolet light , microbiology and biotechnology , dna glycosylase , genetics , chemistry , dna , gene , photochemistry
Since mitochondria are known to leave cyclobutane pyrimidine dimers (CPDs) unrepaired after ultraviolet light exposure, we introduced a mitochondria‐targeted pyrimidine glycosylase (MTS‐pdg) into keratinocytes and thereby initiated mtDNA repair after ultraviolet exposure. We generated stable clonal cell lines with which we are testing whether persistent mtDNA damage may lead to aberant mitochondrial function or cycling. After UV‐B exposure, mtDNA is repaired by MTS‐pdg, and MTS‐pdg expression leads to alterations in cytokine production and mtDNA content after UV‐B exposure. These results confirm that mtDNA plays a role in cellular response to ultraviolet light and may provide clues as to the mechanism by which mitochondrial damage alters cellular processes.