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Role of the Recombination Enhancer in mating‐type switching in budding yeast
Author(s) -
Mehta Anuja,
Haber James
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.542.7
Subject(s) - homologous recombination , gene conversion , saccharomyces cerevisiae , biology , dna , enhancer , genetics , recombination , homology directed repair , dna repair , mating type , homologous chromosome , non homologous end joining , microbiology and biotechnology , mating of yeast , gene , dna mismatch repair , gene expression
DNA double‐strand breaks (DSB) can be repaired by two major processes: nonhomologous end‐joining and homologous recombination. In budding yeast, repair of DSB repair occurs by gene conversion (GC) if both ends of the DSB share homology with another DNA sequence. The Saccharomyces cerevisiae mating‐type switching includes formation of a controlled DSB at the MAT locus and repair from one of two donors, HML and HMR, through the GC repair mechanism. Donor preference has been shown to be highly biased so that MAT a cells recombine preferentially with HML α and MAT α cells with HMR a . This bias is determined by a ~700 bp, cis ‐acting region called the Recombination Enhancer (RE). Preliminary experiments show that in the absence of RE, initiation of DNA synthesis and subsequent repair of the break is strikingly delayed. We propose that the RE region plays an important role in regulating kinetics of MAT switching.