Premium
POSTMORTEM TIME AFFECTS THE INTEGRITY OF DNA EXRACTED FROM THE BRAIN AND KIDNEY OF MALE ALBINO SPRAGUE DAWLEY RATS
Author(s) -
EBUEHI ALBERT O,
BALOGUN Ayooluwa,
Adenike FOWORA,
EBUEHI Olufunke,
AMODE Tolani,
OJIEH Godwin
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.542.2
Subject(s) - dna , biology , nuclear dna , real time polymerase chain reaction , genomic dna , kidney , polymerase chain reaction , microbiology and biotechnology , genetics , gene , mitochondrial dna
When an organism dies, internal nucleases existing within the cells are expected to cause DNA to degrade into smaller fragments over time that will eventually lead to alteration in the profile of the genomic structure of the organism. This study to evaluate the effect of post‐mortem time on the integrity of DNA extracted from the brain and kidney of male Sprague Dawley albino rats. Deoxy ribonucleic acid (DNA) was extracted at different post‐mortem times (0, 24, 48 hours), from the brain and kidney of the male albino rats sacrificed via cervical dislocation. Random amplification of polymorphic DNA Polymerase chain reaction (RAPD‐PCR) was used to detect the effect of post‐mortem time on the integrity of DNA by creating the current profile of the DNA at each post‐mortem time. The post‐mortem DNA profile observed in this study reveals that DNA degradation is a sequential time dependent process. There is a relationship between degradation rate of nuclear DNA and post‐mortem time in the studied organs. The rat brain showed a slower degradation rate and is therefore considered as a valuable organ for studying DNA in longer post‐mortem time. The finding from this study is very useful in forensic science and investigation.