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“Sex‐specific microRNA networks in cardiac hypertrophy”
Author(s) -
Burton Tyler,
Malone Payton,
Baute Audrey,
Waikel Rebekah
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.542.15
Subject(s) - left ventricular hypertrophy , microrna , muscle hypertrophy , cardiac hypertrophy , medicine , heart failure , disease , cardiology , endocrinology , biology , blood pressure , gene , genetics
Sex differences in cardiovascular disease, susceptibly and progressively, including left ventricular hypertrophy (LVH) and subsequent heart failure, have been well documented. MicroRNAs (miRNAs), which have been shown to be regulated during LVH, might provide insight into these sex differences. To establish sex‐specific miRNA profiles in LVH, male and female C57BL6 mice were subjected to transaortic constriction (TAC). Two‐weeks postsurgery, mice were euthanized and heart weights were determined. Male mice had significantly greater levels of cardiac hypertrophy as compared to female mice. Total RNA was isolated from left ventricular tissue samples, and next generation sequencing was performed on small RNAs. Comparing miRNA expression data from TAC, sham surgical, and non‐surgical animals, we have established unique sex‐specific miRNA expression profiles in left ventricular hypertrophy and in a non‐surgical state. We have used these profiles to establish potential signaling networks responsible for sex‐differences. Functional significance of networks known to influence cardiac hypertrophy, including TGFβ and MCIP1, are currently being investigated. It is anticipated that establishment of sex‐specific miRNA networks will aid in the use of miRNAs as diagnostic and therapeutic targets for LVH. NIH 5P20RR016481–12, 8P20GM103436–12, R15HL108192–02