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Differential Replication Fork Progression during Drosophila Follicle Cell Gene Amplification
Author(s) -
Alexander Jessica L,
OrrWeaver Terry L
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.541.6
Subject(s) - amplicon , gene duplication , biology , fork (system call) , genetics , chromatin , dna replication , gene , polymerase chain reaction , computer science , operating system
Progression of replication forks must be tightly monitored to ensure complete duplication of the genome. However, specific features of the chromatin that influence fork progression are still largely unknown. The Drosophila Amplicons in Follicle Cells (DAFCs) present an ideal model system to track fork movement in real‐time and delineate causes of differential fork progression. The DAFCs undergo gene amplification by repeated origin firing and rely on the same machinery that drives DNA replication during S‐phase. Each DAFC reaches a unique level of amplification that reflects the total number of origin firings. Bidirectional fork movement generates a gradient of amplification that spans 100kb at each amplicon. Comparative Genome Hybridization analysis reveals fork progression is not uniform throughout the amplified domain, suggesting local chromatin features affect fork movement. Additionally, ectopic DAFCs generated by P ‐element transformation are subject to position effects influencing the level of amplification. We will present experiments testing whether these position effects also impact replication fork progression and the extent of the amplified domain.