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Sequence Analysis of PTEN in Castanea dentata and PINK1 in Caenhorabditis elegans
Author(s) -
DeGennaro Torrie Lynn,
Dwyer Megan Marie,
Gallagher Madison Elizabeth,
Korolyshun Rachel,
Riccardi Olivia Elizabeth,
Paolella Mary Jane
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.538.6
Subject(s) - pten , pink1 , tensin , genbank , biology , computational biology , genetics , gene , pi3k/akt/mtor pathway , autophagy , signal transduction , apoptosis , mitophagy
This study analyzes the sequences of PTEN (phosphatase and tensin homologue) and PINK1 (PTEN induced putative kinase 1) in Castanea dentata , American Chestnut, and Caenorhabditis elegans respectively. Their genetic mutations can be the cause of breast cancer and Parkinson's Disease. PTEN provides instructions for making a protein found in all tissues of the body and PINK1 furnishes the instructions for making PTEN. The American Chestnut (provided by the CT Agricultural Station) has been subjected to blight in the eastern forests. The worms (obtained from Cold Spring Harbor/DNA Learning Center) share fundamental cellular and molecular structures with humans. A variety of DNA extraction methods were employed including FTA cards, Extract‐n‐Amp, DNeasy kits and specific lab protocols developed for the chestnut by Jeanne Romero‐Severson (U. Notre Dame) and for the worms by James Lissemore (John Carroll University). PCR was performed using student‐designed primers and protocols after careful alignment of genetically similar organisms. The results were then used as templates to sequence the genes on the school‐owned ABI Prism 310 Genetic Analyzer and analyzed using NCBI and Ensembl bioinformatics tools. The results will be submitted to GenBank in June..