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UmuD participates in a primitive DNA damage checkpoint by interacting with DNA pol III α and SSB
Author(s) -
Silva Michelle Cristine,
Chaurasiya Kathy R,
Ruslie Clarissa,
Voortman Lukas,
Ronayne Erin A,
Nevin Philip,
Lone Samer,
Chan Monyrath,
Williams Mark C,
Beuning Penny J
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.538.3
Subject(s) - dna damage , sos response , dna polymerase , dna replication , biology , dna polymerase ii , dna , g2 m dna damage checkpoint , microbiology and biotechnology , dna clamp , dna repair , polymerase , genetics , gene , cell cycle checkpoint , cell cycle , polymerase chain reaction , reverse transcriptase
The α subunit of DNA polymerase III (DNA pol III) is the main replicative polymerase in Escherichia coli . Because α cannot copy damaged DNA, replication is stalled in presence of DNA lesions. To compensate, the cell initiates the SOS response, inducing the expression of at least 57 genes. The products of these genes are involved in DNA damage tolerance mechanisms, one of which is translesion synthesis (TLS), a process by which lesions are bypassed by specialized DNA polymerases with the ability to copy damaged DNA. Full‐length UmuD, whose expression is regulated by the SOS response, inhibits DNA replication and prevents mutagenic TLS as part of a primitive DNA damage checkpoint, while the cleaved form UmuD’ facilitates mutagenesis. It has been suggested that UmuD participates in a primitive DNA damage checkpoint but its exact contribution is still unclear. In order to understand the role of UmuD in regulating DNA replication, the interactions between UmuD and α and between UmuD and SSB were investigated, the latter of which is a novel interaction. As a result, we have determined that UmuD disrupts the interaction between α and the β clamp, as well as the interaction between α and ssDNA, suggesting a direct role for UmuD in disrupting replication. The UmuD‐SSB interaction may contribute to the transition between the replication of undamaged DNA and TLS. Supported by NSF.