Premium
Heart valve substitute fabricated from silk protein, collagen, and poly‐glycerol sebacate has enhanced endothelial cell growth and reduced thrombogenicity
Author(s) -
Wagner William D,
Wang Rui,
LeviPolyachenko Nicole
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.527.4
Subject(s) - nanofiber , platelet , heart valve , materials science , fibroin , platelet activation , thrombogenicity , adhesion , biomedical engineering , biophysics , silk , chemistry , composite material , surgery , immunology , medicine , biology
Mechanical or collagen based porcine valves currently used to replace diseased or nonfunctional valves are subject to thrombosis, material failure, or inadequate in situ remodeling. In this study a new electrospun nanofiber composite was fabricated from mechanically robust silk fibroin, (F), Type I collagen (C), and the synthetic elastic polymer, poly (glycerol‐sebacate) (P). Materials fabricated with different weight ratios of F:C:P had an elastic modulus between 2.8–4.1 Mpa; tensile stresses from 1.1–1.5 Mpa and strains between 41–44% which were similar to values reported for native heart valve. In vitro degradation demonstrated <0.3% mass loss per week with no change in nanofiber diameter. In culture electrospun nanofibers mats of FCP compared to structurally similar C nanofibers had greater human umbilical vein endothelial cell growth and by confocal microscopy demonstrated tight intercellular junctions. Using human platelet rich plasma reduced platelet numbers were observed for FCP (3440 ± 302) (mean ± SEM, platelets/cm 2 )mats compared to C (5268 ± 848) and collagen gels (27,549 ± 698). By SEM, platelets appeared significantly less activated on FCP. Materials preseeded with cells had platelet adhesion of 555 ± 137, 1588 ± 41, and 12311 ± 208 respectfully for FCP, C, and collagen gels. The findings suggest FCP may be a superior material for heart valve replacement.