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Francisella tularensis does not alter ubiquitylated or SUMOylated proteins profiles during epithelial cell infections
Author(s) -
Chua Michael,
Guttman Julian A.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.523.7
Subject(s) - francisella tularensis , francisella , sumo protein , biology , ubiquitin , microbiology and biotechnology , cell , intracellular , virulence , genetics , gene
Francisella tularensis are intracellular bacteria that cause tularaemia, an infection that can lead to 60% mortality rates if left untreated. Although these microbes infect both macrophages and epithelial cells, the epithelial cell infections are known to be crucial for the disease process. A variety of bacteria alter the normal processes of post‐translational protein modifications that occur in healthy epithelial cells. These include the processes of ubiquitylation and SUMOylation. To begin to determine the host cell alterations that occur during Francisella ‐based epithelial cell infections, we hypothesized that ubiquitylation and/or SUMOylation alterations to host proteins would be altered during the infections. To test this hypothesis, we analyzed protein immunoblots of two cell lines, HeLa and COS‐7, that we infected with Francisella for 24 hours. Comparison of uninfected and infected samples showed no significant differences in ubiquitin, SUMO1 or SUMO2/3 modifications to any host cell proteins. Through our study, we concluded that Francisella does not alter the host ubiquitin or SUMO post‐translational protein modifications as part of their disease mechanism. This study was funded by CIHR.Grant Funding Source : Canadian Institutes of Health Research