Premium
Nanoenhanced Hydrogel System with Sustained Release Capabilities for Bone Regeneration
Author(s) -
Karnik Sonali,
Hines Kanesha,
Mills David
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.521.5
Subject(s) - self healing hydrogels , halloysite , biomedical engineering , regeneration (biology) , tissue engineering , mineralization (soil science) , chemistry , implant , controlled release , materials science , nanotechnology , microbiology and biotechnology , surgery , polymer chemistry , composite material , biology , medicine , organic chemistry , nitrogen
Tissue engineering strives to integrate structure and functionality in bioengineering scaffolds or implants. These materials must be biodegradable, immunocompatible, and integrate well with the natural tissue. The bioengineered scaffolds and implants should also be eco‐friendly to manufacture. The aim was to design an implant that enhances inherent bone regeneration y, mimics the natural internal environment and is composed of eco‐friendly materials. We used halloysite nanotubes (HNTs), naturally occurring nanoclay, similar to the kaolin chemically and structurally, and composed of oppositely charged concentric layers of hollow tubules. This structure enables HNTs to acts as a nanocontainer and release bioactive molecules periodically and in a sustained manner. We combined HNTs with alginate hydrogels to release BMPs 2, 4, and 6 and are known to induce osteogenic expression in osteoblasts. We obtained the sustained release of BMPs over a seven‐day period. The amount released was in the range of picograms per milliliter. Osteoblasts encapsulated in these nanoenhanced hydrogels showed enhanced mineralization and ECM secretion as compared with controls over a period of 28 days. Cellular response varied depending on BMP treatment. This nanoenhanced hydrogel system may be a viable solution for in situ bone regeneration for use in degenerative joint disease, small fractures and other bone defects.