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Important techniques in today's biomedical science research that African PhD candidates should be exposed to: a perspective from the FASEB Journal
Author(s) -
John Theresa Adebola
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.516.3
Subject(s) - multidisciplinary approach , computational biology , biology , library science , microbiology and biotechnology , computer science , sociology , social science
The need for best evidence for profitable research has necessitated multidisciplinary, collaborative approaches which are mainstay in today's biomedical science (BS). For African PhD graduates in basic medical sciences to better profit from research, they need multidisciplinary approaches to research during their subject‐based training. The present report sought to substantiate this. Thirty three published articles in the April 2012 FASEB journal were studied for the methods employed. The papers utilized an average of 9 major BS techniques, 9 being the mean, median, and mode showing the global status quo of diversity of methodology per scientific paper. The most popular procedures and techniques recorded in more than 1/3 of the articles were: cell isolation; cell culture; in vivo or in situ whole animal studies; animal models of disease; gene/protein expression, sequencing and cloning; transfection, constructs, and genomic interference and silencing; western blotting; fluorescence and confocal microscopy; ELISAs and cell‐based assays; and ready‐made biotech assay kits. The most popular statistics were various student's t‐tests at 0.05 confidence levels and ANOVAs. The GraphPad Prism was the most frequently used statistic software. These methods are therefore recommended to be included in Ph.D. training.