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Autologous subcutaneous adipose tissue transplant in diet induced obese rats.
Author(s) -
TorresVillalobos Gonzalo Manuel,
HamdanPérez Nashla,
Torres Nimbe,
OrdazNava Guillermo,
MoranRamos Sofia,
Noriega Lilia,
BravoReyna Carlos,
TorreVillalvazo Ivan,
Hiriart Marcia,
MedinaSantillan Roberto
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.48.5
Subject(s) - adipose tissue , triglyceride , medicine , endocrinology , transplantation , obesity , subcutaneous tissue , weight loss , cholesterol , surgery
Obesity is a complex disease Objective Evaluate autologous subcutaneous adipose tissue (SAT) functionality as a therapeutic measure for obesity and its comorbidities. Methods We feed Wistar rats with a high‐carbohydrate (HC) or a high animal fat (HF) diet for 90 days. Glucose and triglyceride (TG) concentrations were evaluated each 30 days, oral glucose tolerance test (OGGT) at days 0, 90 and 180. On day 90 surgical procedures were performed randomly on all rats: 1) Sham (S) control, 2) autologous SAT transplant to visceral adipose tissue (T), 3) extraction (E) of SAT. Results On day 180 OGGT showed lower insulin concentrations on T group with HF diet (p =0.002) in comparison to S, as well as a smaller increase in groups T and E on both diets (p=0.004) compared to S. The group with less weight gain was T with both diets. At day 180 TG concentrations were lower in groups E and T of both diets compared to group S. We measured cholesterol and TG in hepatic tissue, it was higher in S and E groups compared with T on HF diet (p=0.008). Rats that underwent autologous SAT transplantation showed a better response during OGGT and a smaller increase in weight compared to S group. Conclusion Autologous SAT transplantation to visceral compartment might be a useful clinical measure to improve carbohydrate metabolism and weight control.