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Dietary macronutrients modulate the fatty acyl composition of rat liver mitochondrial cardiolipins
Author(s) -
Stavrovskaya Irina G,
Bird Susan S.,
Marur Vasant R.,
Sniatynski Matthew J.,
Baranov Sergei V.,
Greenberg Heather K.,
Porter Caryn L.,
Kristal Bruce S.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.48.4
Subject(s) - cardiolipins , mitochondrion , biochemistry , chemistry , lipogenesis , bioenergetics , lipidomics , cardiolipin , medicine , endocrinology , biology , metabolism , phospholipid , membrane
Cardiolipins (CLs) are a family of phospholipids essential for mitochondrial structure and function. The defective remodeling of the CL molecules (i.e., the changing of the fatty acyl [FA] composition) has been linked to human pathology. We examined diet‐mediated CL changes in liver mitochondria from healthy male FBNF1 rats fed 24 different low‐fat, isocaloric diets. Growth rates and mitochondrial respiratory parameters were essentially unaffected, but mass spectrometry‐based, mitochondrial lipidomics profiling revealed up‐regulation of CLs in rats fed saturated or trans fat‐based diets with a high glycemic index. These mitochondria showed elevated monolysocardiolipins and decreased ubiquinone Q 9, implying a low‐grade mitochondrial redox abnormality and compensatory CL elevation. Extended analysis of the rat liver mitochondrial CL pools has revealed previously unobserved regulation of CL, suggesting that (i) dietary fats and, to a lesser extent, carbohydrates induce changes in the relative abundance of CL species; (ii) FA incorporation into mature CLs undergoes both positive (~400‐fold) and negative (≥2.4‐fold) regulation; (iii) dietary lipid abundance and incorporation of FAs into both the CL pool and specific mature tetra‐acyl CLs are inversely related, suggesting compensatory regulation. This study was funded by U01‐ES16048 (BSK, PI) and P30‐ DK040561 (W. A. Walker, PI).