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Higher‐order chromatin folding and gene regulation
Author(s) -
Bickmore Wendy
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.456.1
Subject(s) - chromatin , histone , nucleosome , biology , chia pet , regulation of gene expression , gene , gene expression , histone modifying enzymes , microbiology and biotechnology , bivalent chromatin , chromatin remodeling , genetics , psychological repression , computational biology
It is widely appreciated that histone modifications correlate to gene expression states and can, in some cases, directly impact on gene expression and repression. But, our understanding of chromatin states beyond the level of the nucleosome itself is much more rudimentary. Until quite recently there has been little exploration of how these levels of higher‐order chromatin structure might contribute to the regulation of gene expression. Using specific developmentally regulated loci in the mouse, I will discuss how chromatin compaction can act to regulate the spatial and temporal activation of genes during mouse development. I will compare different methods that can probe higher order chromatin structure and will demonstrate their use in both cell culture systems that reflect developmental stages, and in embryos.