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Relationship between human milk oligosaccharides and fecal microbiome of breastfed infants
Author(s) -
Williams Janet E,
Riley Mara A,
Brooker Sarah L,
Hunt Katherine M,
Szyszka Alexandra,
Bode Lars,
McGuire Michelle K,
McGuire Mark A
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.45.5
Subject(s) - feces , leuconostoc , bacteroides , weissella , biology , microbiome , relative species abundance , abundance (ecology) , lactococcus , food science , microbiology and biotechnology , streptococcus , pyrosequencing , bacteria , lactococcus lactis , genetics , ecology , lactobacillus , gene , lactic acid , fermentation
Human milk oligosaccharides (HMO) have been shown to impact growth of staphylococci and bifidobacteria in vitro . However, little is known as to whether amounts of HMO consumed by infants are related to the composition of their gastrointestinal (GI) microbiomes. To examine this, milk and infant fecal samples were collected from mother/infant dyads ( n = 12) on d 2, 5, 10, 30 and 60 after birth. DNA was extracted, the 16S rRNA gene amplified, and relative microbial abundance characterized by 454 pyrosequencing. HMO concentrations were determined using HPLC. Spearman's correlation coefficients were calculated to relate HMO concentration with relative abundance of fecal bacterial genera and diversity indices at each time point. On d2, Streptococcus abundance was negatively correlated (r = −0.94) with 2′fucosyllactose (2′FL); Lactococcus , Leuconostoc , and Weissella abundances were correlated (r = 0.98, 0.92, and 0.93, respectively) with disialyllacto‐N‐tetraose (DSLNT). On d10, Bacteroides abundance was correlated (r = 0.73) with 2′FL. On d2, DSLNT correlated (r = 0.88) with the Chao richness index. Results suggest possible associations between HMO and presence of some microbial genera, but relationships were not consistent over time. Supported by the Bill and Melinda Gates Foundation and the Institute for Bioinformatics and Evolutionary Studies (IBEST).