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The acute aerobic exercise‐induced increase in amino acid transporter expression adapts to exercise training in older adults
Author(s) -
Markofski Melissa M,
Timmerman Kyle L,
Dickinson Jared M,
Reidy Paul T,
Borack Michael,
Rasmussen Blake B,
Volpi Elena
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.350.3
Subject(s) - sarcopenia , medicine , placebo , aerobic exercise , transporter , ex vivo , endocrinology , physical therapy , biology , biochemistry , pathology , gene , alternative medicine , in vitro
Exercise and nutritional interventions may prevent or delay frailty and sarcopenia in older adults. The OBJECTIVE of this ongoing study is to determine the acute and chronic effects of aerobic exercise +/− essential amino acid (EAA) supplementation on skeletal muscle amino acid (AA) transporter expression. Preliminary data are reported. METHODS Healthy untrained older adults (65–85 y; n=10) were randomized into one of two groups: exercise and 15 g EAA supplement (Ex+EAA) or exercise and placebo (Ex+Pla). Acute treatment consisted of 45 mins of walking exercise on a treadmill at ~70% heart rate max (Ex+EAA and Ex+Pla) immediately followed by the assigned supplement or placebo. Chronic treatment involved once daily supplementation (EAA or Pla) plus aerobic exercise 3 times/wk. Real‐time qPCR was used to measure mRNA expression of select muscle AA transporters. RESULTS At baseline, CAT1, SNAT2, PAT1, CD98, and LAT1 mRNA expression increased more after Ex+EAA than Ex+Pla. After chronic treatment, only CD98 increased with Ex+EAA. There were no basal pre to post changes. CONCLUSION Muscle AA transporter expression increases acutely with Ex+EAA. Exercise training+EAA induces adaptations reducing the acute AA transporter response to these stimuli. Supported by NIH/NIA R01 AG030070 and NIH/NIA R01 AG030070S1 Grant Funding Source : NIH