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Polyphenols and phenolic acids modulate inflammatory cytokine release by Jurkat human CD4+ T‐cells
Author(s) -
Ford Christopher Thomas,
Richardson Sian,
McArdle Francis,
Crozier Alan,
McArdle Anne,
Jackson Malcolm J
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.348.5
Subject(s) - jurkat cells , cytokine , tumor necrosis factor alpha , polyphenol , chemistry , pharmacology , quercetin , phorbol , cell culture , proinflammatory cytokine , t cell , biochemistry , inflammation , immunology , medicine , biology , immune system , signal transduction , protein kinase c , antioxidant , genetics
Altered circulating levels of inflammatory cytokines are a risk factor for the development of age related diseases. The objective of this study was to assess the effect of polyphenols or phenolic acids on the release of cytokines from a human CD4+ T‐cell line (Jurkat cells). Cells were treated with a range of polyphenols or phenolic acids at 1 μM or 30 μM. Cells were treated with phorbol 12‐myristate 13‐acetate (PMA) stimulation after 24 hours and cytokine release was determined 24 hours later. Four compounds were identified that resulted in a significant reduction in interleukin 2 (IL‐2) and tumour necrosis factor α (TNFα) release by PMA‐stimulated Jurkat cells. These molecules were dihydroferulic acid (−56.5% TNFα, p<0.001), feruloylglycine (− 49.9% TNFα, p<0.001), quercetin (−67.2% IL‐2, p<0.001; −91.8% TNFα, p<0.001), and 3‐O‐methylquercetin (−91.5% TNFα, p<0.001). A further 3 compounds, epigallocatechin gallate (−46.2% IL‐8, p<0.01), punicalagin (−43.1% IL‐8, p<0.05), and callistephin (−48.5% IL‐8, p<0.01), reduced the release of cytokines by non PMA‐stimulated Jurkat cells. In conclusion, we have identified 7 dietary polyphenols or phenolic acids that reduced the release of inflammatory cytokines by a human CD4+ T‐cell line. The authors thank the British Biological Sciences Research Council Diet and Health Research Industry Club (BBSRC DRINC) programme for financial support.