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Polyphenol‐rich red and purple aronia berry extracts inhibit interleukin‐6 from mouse splenocytes.
Author(s) -
Martin Derek,
Taheri Rod,
Brand Mark,
Draghi Andrew,
Sylvester Francisco,
Bolling Bradley
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.348.3
Subject(s) - polyphenol , proanthocyanidin , berry , chemistry , anthocyanin , phenols , flavonoid , splenocyte , food science , acetone , traditional medicine , botany , biochemistry , in vitro , biology , medicine , antioxidant
Viking aronia berries are a rich source of dietary polyphenols, but less is known about other aronia genotypes. The objective of this work was to assess the anti‐inflammatory activity of underutilized aronia berries to relative to Viking. Aqueous acetone extracts were prepared from Aronia melanocarpa (black) , prunifolia (purple), and arbutifolia (red) berries (n = 5). Extract polyphenols were quantitated by UPLC‐MS and the total phenols assay. Primary mouse splenocytes were stimulated for 12 h by LPS with berry extracts and interleukin‐6 (IL‐6) was determined in media. On an equivalent total phenols basis, Viking had at least 1.5‐fold greater anthocyanins and proanthocyanidins than other accessions. Phenolic acids were highest in the purple UCO47 and red UCO57 accessions. The UCO57 extract inhibited IL‐6 by 75% and was the most potent among the tested aronia extracts. In contrast, Viking inhibited IL‐6 by 32%. Among all aronia accessions, IL‐6 inhibition was correlated more strongly to phenolic acid and flavonoid content than proanthocyanidin and anthocyanin content. Thus, underutilized aronia accessions have in vitro anti‐inflammatory efficacy, and are promising candidates for evaluation in models of chronic disease. Grant Funding Source : University of Connecticut Diet and Health Initiative