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Anti‐inflammatory activities of Lessertia frutescens (Sutherlandia) extract in murine macrophages
Author(s) -
Lei Wei,
Browning Jimmy,
Eichen Peggy Ann,
Lu ChiHua,
Folk William,
Sun Grace,
Fritsche Kevin
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.348.2
Subject(s) - tlr5 , tlr2 , chemokine , tlr4 , nitric oxide , chemistry , macrophage , toll like receptor , nitric oxide synthase , pharmacology , receptor , biology , biochemistry , innate immune system , in vitro , organic chemistry
Introduction Lessertia frutescens (Sutherlandia) is a medicinal plant native to southern Africa and is traditionally used in various inflammatory situations. We sought to characterize the bioactivity of an ethanolic extract from Sutherlandia (SE) using a murine macrophage cell line (RAW 264.7) and primary murine macrophages. Experimental Design Cells were exposed to various concentrations of SE for 30 min, then stimulated with IFNγ along with various toll‐like receptor (TLR) ligands for 20 hours. The production of nitric oxide (NO), several pro‐inflammatory cytokines/chemokines, as well as the expression of iNOS protein was evaluated 20 hr later. Results SE treatment significantly and dose‐dependently reduced NO production induced by the TLR5 ligand, flagellin, TLR4 ligand, LPS, and TLR2/6 ligand, Pam2CSK. Similar effects were observed for RAW 264.7 cells and primary murine macrophages. The impact of SE on iNOS protein expression was consistent with the observed reductions in NO production. SE treatment also diminished the production of various pro‐inflammatory cytokines and chemokines upon stimulation of macrophages with IFN‐γ and TLR ligands. Conclusions Sutherlandia ethanolic extract has potent anti‐inflammatory activities in murine macrophages. Funding from the Office of Dietary Supplement, NIH: P50 AT006273 ‐01. Grant Funding Source : ODS, NIH