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Protein turnover in pregnant pigs at amino acid intake in excess of requirements
Author(s) -
Moehn Soenke,
Rafii Mahroukh,
Pencharz Paul B,
Ball Ronald O
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.345.8
Subject(s) - gestation , pregnancy , phenylalanine , amino acid , medicine , protein turnover , chemistry , zoology , endocrinology , biochemistry , biology , protein biosynthesis , genetics
Ten experiments to determine amino acid (AA) requirements of pigs in early and late pregnancy were evaluated to determine which factors affected protein turnover when the test AA intake was above requirements. In all experiments, each of 6 to 7 sows were fed 6 diets with graded levels of test AA in both early and late gestation. Protein turnover was determined based on 13C enrichment after oral L[1–13C]phenylalanine (Phe) dosing using a stochastic model. The observations (max n=200) were evaluated using mixed models (SAS). The final models fit the data with r2 = 0.79 (protein synthesis, S) to r2 = 0.87 (Phe oxidation, Ox, g/d). Greater AA and Phe intake increased (P < 0.05) S, breakdown (B) and Phe flux. Ox and B decreased as pregnancy progressed (P < 0.02), while S, flux and Phe retention (RPhe) increased (P<0.08). Interactions (P<0.02) between AA intake, Phe intake and gestational age increased S, flux and RPhe, indicating a need for more AA towards term. Greater metabolizable energy (ME) intake increased flux (P = 0.003) but decreased B (P = 0.001) in interaction with AA intake (P < 0.02). Thus, pregnant sows can use more AA at higher ME intake. Ox was greatest (P = 0.001) in the 3rd parity but was reduced (P < 0.03) by interactions with increasing BW, ME intake and gestational age. Thus, Phe kinetics may change from adolescent to adult sows. More AA and ME than stated in requirements may be needed close to term when sows carry large litters.