Premium
A prospective study of global DNA methylation and development of adiposity in Colombian schoolchildren
Author(s) -
Perng Wei,
MoraPlazas Mercedes,
Rozek Laura S.,
Marin Constanza,
Baylin Ana,
Villamor Eduardo
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.343.1
Subject(s) - quartile , dna methylation , methylation , body mass index , anthropometry , waist , medicine , demography , cohort , prospective cohort study , endocrinology , biology , genetics , confidence interval , dna , gene expression , gene , sociology
Objective To examine associations of global DNA methylation with changes in adiposity in a longitudinal study of school‐age children from Bogotá, Colombia. Methods We quantified methylation of LINE‐1 elements from peripheral leukocytes of 553 children aged 5–12 y at baseline using pyrosequencing technology (Qiagen). Anthropometric characteristics were measured periodically for a median of 30 months. We estimated mean change in 3 age‐standardized indicators of adiposity: body mass index (BMI) Z score (BMIZ), waist circumference Z score (WCZ), and triceps‐to‐subscapular skinfold thickness ratio Z score (SKFZ) according to quartiles of LINE‐1 methylation using multivariable mixed effects linear regression. Results There were inverse, non‐linear relations of DNA methylation with BMIZ and SKFZ change in boys. After adjustment for baseline age and socioeconomic status, boys in the lowest quartile of LINE‐1 methylation experienced annual gains in BMIZ and SKFZ that were 0.06 Z/y ( P =0.04) and 0.07 Z/y ( P =0.03), respectively, higher than those in the upper three quartiles. There was an inverse, monotonic relation of LINE‐1 methylation with annual change in WCZ ( P trend=0.02). DNA methylation was not related to development of adiposity during follow‐up among girls. Conclusions Global DNA methylation was inversely related to development of central adiposity in boys. Grant Funding Source : This study was funded by the Robert Wood Johnson Foundation Research Project Awards. The Bogotá School Children Cohort is currently sponsored by the ASISA Research Fund at the University of Michigan.