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mRNA surveillance is driven by translation
Author(s) -
Green Rachel,
Guydosh Nicholas
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.325.3
Subject(s) - ribosome profiling , nonsense mediated decay , translation (biology) , ribosome , messenger rna , biology , microbiology and biotechnology , protein biosynthesis , computational biology , gene , genetics , rna , rna splicing
There are several mRNA surveillance pathways in eukaryotes that moderate the effects of natural errors in the cell and more broadly regulate gene expression. We have recently defined biochemical parameters of the no‐go decay quality control system using our previously developed in vitro reconstituted yeast translation system. In these studies, we have shown that two factors implicated in no‐go decay (NGD) and non‐stop decay (NSD), Dom34 and Hbs1, promote subunit dissociation and peptidyl‐tRNA drop‐off on the ribosome to initiate recycling events that eventually lead to mRNA decay. Of particular interest is that these factors (Dom34 and Hbs1) appear to identify stalled ribosome complexes that are associated with 3’ truncated mRNAs, suggesting a biochemical outcome for a likely in vivo consequence of stalling during translation (endonucleolytic cleavage of the mRNA). Current efforts focus on a broad analysis of the in vivo targets of mRNA surveillance (including NGD, NSD and NMD) using a series of reporter constructs and genome wide ribosome profiling.