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Nondestructive microCT imaging of soft tissues using phosphotungstic acid‐hematoxylin, intravascular contrast agent, and perfluorochemical
Author(s) -
Barbe Mary F,
Phillips Steve J,
Ruggieri Michael R,
Miller Larry S,
Wolfson Marla R
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.317.2
Subject(s) - parenchyma , h&e stain , lung , pathology , soft tissue , staining , medicine , histology , contrast (vision) , anatomy , artificial intelligence , computer science
Nondestructive imaging of soft tissues can be an important tool experimentally. However, the low X‐ray attenuation of most soft tissues renders them nearly invisible in radiographs. Whole tissue staining with phosphotungstic acid‐hematoxylin (PTAH) in conjunction with micro‐CT will be discussed for visualizing cartilaginous structures in animal fetuses. Combining PTAH with microCT will be discussed for examining the anatomic orientation and mean cross‐sectional thickness of muscle components of the gastro esophageal junction components in human organ donor specimens (50 mm3 in size), findings confirmed in patients with endoscopic ultrasonography. Whole lung staining with PTAH in combination with Microfil (an intravascular contrast agent) and then microCT will be presented for segmenting lung parenchyma from microvasculature in rodents for quantitative morphometry. Also, the use of perfluorochemical (PFC) to fill lung airways combined with Microfil and then microCT will be presented. Since PFC is radiopaque, its concentration can be adjusted to different Houndsfield units than Microfil to assay bronchial/alveolar volumes separately from microvasculature and lung parenchyma volumes.