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Imaging neurovascular interactions in the cerebral cortex in vivo
Author(s) -
Devor Anna
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.313.3
Subject(s) - neuroscience , premovement neuronal activity , in vivo , neurovascular bundle , optogenetics , vasodilation , biology , anatomy , endocrinology , microbiology and biotechnology
The ability to descend to the single‐cell and single‐capillary levels in vivo and observe firing of individual neurons, vasodilation, glucose uptake, and infusion of O 2 into the tissue ‐ all while directly controlling neuronal activity ‐ has long been a dream of scientists interested in understanding the complex regulation of blood flow and metabolism as related to neuronal activity. However, in contrast to the detailed and elegant mechanistic studies in isolated tissue, in vivo reports have, in the main, focused simply on correlations between the “observables”, limited by the available methods. This “too hard to do” status quo for mechanistic studies in vivo is starting to change, due to rapid developments in optical microscopy. In fact, already today, a versatile suite of optical tools is available for high‐resolution, high‐sensitivity measurements of vascular, metabolic, and neuronal parameters in deep tissue and local, cell‐type specific manipulations of neuronal activity. In this talk, we will review examples where the use of novel optical technologies has been instrumental for central discoveries in basic mechanisms of neurovascular regulation.